WESTERN IMMUNOBLOT ANALYSIS OF TWENTY-ONE SNAKE VENOMS FROM THREE SNAKE FAMILIES

Authors
  1. Berger, B.J.
  2. Bhatti, A.R.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Snake venom components antigenically similar to those found in Vipera russelli venom was investigated across a wide spectrum of species. A polyvalent antiserum against Vipera russelli venom was raised in rabbits, and used in Western immunoblotting to probe nitrocellulose filters that held SDS-PAGE-size-separated snake venom proteins from twenty one different species, ranging from other Vipera ssp. to Naja spp. It was found that all species, even those distantly related to Vipera russelli, contained at least one immunologically cross-reacting component. The elapid samples were found to contain some of the strongest cross-reactive components, while many of the more closely related snakes showed weaker cross-reactivity. There appeared to be no correlation between the intensity and number of cross-reactive bands, and the relative evolutionary distance from Vipera russelli.
Report Number
DRES-520 —
Date of publication
15 May 1989
Number of Pages
23
DSTKIM No
90-02772
CANDIS No
65030
Format(s):
Hardcopy;Originator's fiche received by DSIS

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