TOPOLOGICAL DISTRIBUTION OF ATROPINE IN DIPALMITOYLPHOSPHATIDYLCHOLINE LIPOSOMES

Authors
  1. Kafalieva, D.
  2. Shek, P.N.
  3. Stanacev, N.Z.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN);Toronto Univ, Toronto ONT (CAN) Dept of Clinical Biochemistry
Abstract
The topography of atropine entrapped in sn-3 (dipalmitoyl)phosphatidylcholine (DPPC) liposomes was determined by the electron spin resonance (ESR) technique using 5-, 7-, and 16-nitroxy-stearic acid probes. The liposome preparations, with or without entrapped atropine, were passed through a Sepharose-4B column; the entrapment efficiency and lipid recovery were determined using (3H)-atropine and (14C)-DPPC as tracers. It was found that approximately 31 per cent of the added atropine was entrapped in the liposomes. The ESR results established that the temperature range of the gel-to-liquid crystalline transition of DPPC liposomes was not altered significantly by the encapsulation of atropine. The entrapped atropine also caused no significant change in the order of hydrocarbon chains of DPPC vesicles. These results were confirmed by data obtained from differential scanning calorimetry. TRUNCATED
Report Number
DCIEM-89-P-51 — Research Paper; Reprint
Date of publication
15 Aug 1989
Number of Pages
9
Reprinted from
J Microencapsulation, vol 7, no 2, 1990, p 219-227
DSTKIM No
90-03542
CANDIS No
65791
Format(s):
Hardcopy;Originator's fiche received by DSIS

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