ANALYSIS OF A MIDDLE EASTERN SOIL SAMPLE FOR CHEMICAL WARFARE AGENTS

Authors
  1. Sawyer, T.W.
  2. Weiss, M.T.
  3. D'Agostino, P.A.
  4. Provost, L.R.
  5. Hancock, J.R.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A soil sample originating from a Middle Eastern area of chemical warfare activity was subjected to chemical and biochemical analysis. Sarin and several related compounds were confirmed in the soil by capillary column GC-MS, however, the binding of these compounds to the soil hindered quantitation. The chemical results were then compared to those obtained by biochemical means in primary cultures of chick embryo forebrain neurons. By comparing the sample's anticholinesterase activity against those of purified standards in chick embryo neuron cultures, a reasonable agreement was found between the chemical and biochemical semi-quantitative estimates of sarin content in the soil extract. Furthermore, the in vitro system appears to offer a sensitive technique for the estimation of sarin remaining bound to the soil following solvent extraction.
Report Number
DRES-M-1310 — Memorandum
Date of publication
15 Jun 1990
Number of Pages
37
DSTKIM No
90-04504
CANDIS No
66737
Format(s):
Hardcopy;Originator's fiche received by DSIS

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