THE ACETYLCHOLINESTERASE INHIBITING ACTIVITY OF ORGANOPHOSPHATE NERVE AGENTS IN PRIMARY CULTURES OF MOUSE EMBRYO NEURONS

Authors
  1. Sawyer, T.W.
  2. Weiss, M.T.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Primary cultures of mouse embryo cortical neurons were examined with respect to their usefulness as indicators of the relative anticholinesterase activities of tabun, soman and VX. The acetylcholinesterase (AChE) activity of mouse cortical neuron cultures was low for the first eight to ten days in vitro (DIV), and then gradually increased until 21 DIV. This closely paralleled AChE development in mouse neonates. Mouse neurons were extremely sensitive to effects of organophosphate nerve agents at all times during the culture period and treatment of these cultures resulted in a reproducible dose-dependent inhibition AChE activity which did not vary significantly with time in culture. The median inhibitory concentrations (IC sub 50) of tabun, soman and VX are reported.
Report Number
DRES-M-1307 — Memorandum
Date of publication
15 Jun 1990
Number of Pages
26
DSTKIM No
90-04507
CANDIS No
66740
Format(s):
Hardcopy;Originator's fiche received by DSIS

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