IMMOBILIZATION OF PROTEINS FOR BIOSENSOR DEVELOPMENT

Authors
  1. Vandenberg, E.T.
  2. Brown, R.S.
  3. Krull, U.J.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);Toronto Univ, Toronto ONT (CAN) Dept of Chemistry
Abstract
There is considerable interest in the permanent attachment of biomolecules to solid surfaces because of the many uses of immobilized proteins: for purification of molecules that bind to antibodies, lectins or other binding proteins; in solid-phase analytical assays; for improved biocompatibility of materials; and to provide selectivity to biosensors. In such applications, the amount of protein immobilized and the retention of physiological activity of the immobilized protein (such as binding of a second molecule) are of interest. This work will report the amount and activity of immobilized proteins as a function of the method of immobilization, using instrumental analytical techniques.
Report Number
DRES-CR-40-90 — Contract Report
Date of publication
01 Jan 1990
Number of Pages
187
DSTKIM No
90-04576
CANDIS No
66809
Format(s):
Hardcopy;Originator's fiche received by DSIS

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