RAPID IDENTIFICATION OF FRANCISELLA TULARENSIS BY A FLUOROGENIC ENZYME IMMUNOASSAY

Authors
  1. Siddiqui, Y.M.
  2. Fulton, R.E.
  3. Knodel, M.H.
  4. Bhatti, A.R.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A highly sensitive fluorogenic enzyme-linked immunosorbent assay (FELISA), which utilizes nitrocellulose membranes as solid phase support and a fluorogenic substrate to indicate the antigen, has been adapted for the rapid identification of Francisella tularensis. Multiple samples were assayed in approximately 6 h by this method. The sensitivity achieved in a four layer "sandwich" assay format was 10 fg mL1(-) of outer membrane protein and 100 colony forming units mL1(-) of Francisella tularensis whole cells. The assay was highly specific for the detection of homologous and heterologous strains of Francisella tularensis while unrelated bacteria, including Brucella strains, exhibited no cross-reactivity.
Report Number
DRES-M-1302 — Memorandum
Date of publication
15 Nov 1990
Number of Pages
36
DSTKIM No
91-00488
CANDIS No
67707
Format(s):
Hardcopy;Originator's fiche received by DSIS

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