PROPHYLAXIS AND TREATMENT OF INFLUENZA A VIRUS INFECTION BY CARRIER-MEDIATED PASSIVE IMMUNITY

Authors
  1. Wong, J.P.
  2. Stadnyk, L.L.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Liposome-mediated passive immunity was evaluated for its efficacy in the prophylaxis and treatment of influenza A/PR/8 virus infection in mice. Avirulent, egg-propagated influenza A/PR/8 virus (H1N1) was adapted for growth in Balb/C mice. In the in vivo protection study, purified polyclonal antibody (PA) which demonstrated strong reactivity against the mouse-adapted virus in an indirect fluorogenic enzyme-linked immunosorbent assay (FELISA) and in an in vitro plaque assay, was encapsulated within liposomes. Using I125-IgG as a radioactive tracer for the antibody molecules, the delivery of antibody to the lungs was optimized by intranasal administration of PA encapsulated within negatively charged multilamellar vesicles made from phosphatidylcholine:cholesterol:phosphatidylserine (7:2:1). TRUNCATED
Report Number
DRES-M-1326 — Memorandum
Date of publication
15 Jan 1991
Number of Pages
34
DSTKIM No
91-01427
CANDIS No
68635
Format(s):
Hardcopy;Originator's fiche received by DSIS

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