IMMUNOASSAY OF MOUSE IMMUNOGLOBULIN G BY A LIGHT-ADDRESSABLE POTENTIOMETRIC SENSOR

Authors
  1. Thompson, H.G.
  2. Lee, W.E.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A sensitive enzyme immunoassay for the quantitation of mouse immunoglobulin G (mIgG) was developed using a light-addressable potentiometric (LAP) sensor as the detection system. The assay was carried out on nitrocellulose membrane filters and used sandwich-format one-step incubations of antigens and antibodies. In addition, the assay employed a high affinity biotin-streptavidin interaction to capture the immunocomplexes onto nitrocellulose membranes, and a urease-conjugated anti-moust IgF detector antibody. The lower limits of detection of mIgG by this assay were 300, 100, and 10 pg per well, respectively, for 1, 5, and 60 min incubations. The time required for the assay was the incubation time plus 4-5 min, in total for the mixing, washing and detection procedures.
Report Number
DRES-554 —
Date of publication
15 Apr 1991
Number of Pages
35
DSTKIM No
91-02522
CANDIS No
69720
Format(s):
Hardcopy;Originator's fiche received by DSIS

Permanent link

Document 1 of 1

Date modified: