Self-assembled nanostructures for bioanalysis

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Authors
  1. Lee, W.E.
  2. Tang, T.
  3. Lin, D.
  4. Mohammed, A.M.
  5. Harrison, D.J.
  6. Jemere, A.B.
Corporate Authors
Defence Research and Development Canada, Suffield Research Centre, Ralston AB (CAN);Alberta Univ, Edmonton ALTA (CAN) Dept of Chemistry
Abstract
During the past two decades the focus of research and development for biodetection and identification has been on sensors. In those works the main steps in bioanalysis, i.e., molecular recognition, separation and signal generation have been considered and dealt with as independent processes. The lack of success for fieldable bioanalysis platforms can be largely attributed to the difficulty in integrating these steps. The report describes a new approach for an automated bioanalysis platform carried out with support from the DRDC Technology Investment Fund during 2012–2014. The goal of the project was to employ nanomaterials that self-assemble in sensors for bioanalysis. The project addressed fundamental problems with sensor systems using principles of good design so that the output technology would be amenable to automated fluidic processing. Design issues were 1) how to fabricate low cost sensors that would be compatible with microfluidic manifolds; 2) how to build a sensor-detection molecular structure from self-assembled building blocks; and 3) how to create better building blocks. The sensor employed micro-fabricated gold electrodes and self-assembling monolayers for insulation and molecular recognition, and electrochemical impedance spectroscopy for all-electronic signal detection. The principal discovery in the work was an electrochemical reductive desorption protocol for in situ regeneration of gold electrodes. The regeneration process allows for repeated use of a sin

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Keywords
self-assembly;sensors;bioanalysis;electrochemical impedance spectroscopy;detection;gold nanoparticles
Report Number
DRDC-RDDC-2015-R084 — Scientific Report
Date of publication
01 May 2015
Number of Pages
34
DSTKIM No
CA040682
CANDIS No
801899
Format(s):
Electronic Document(PDF)

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