Post-exposure prophylaxis against western equine encephalitis virus by adenovirus-mediated delivery of interferon-alpha gene

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Authors
  1. Wu, J.Q.H.
  2. Barabé, N.D.
  3. Huang, D.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
Western equine encephalitis virus (WEEV) is an endemic and biowarfare agent which poses a potential threat to the operations of the Canadian Armed Forces (CAF). A vaccine for the prevention of WEEV infection before exposure to the virus has been developed and approved by the U.S. Food and Drug Administration as an investigational new drug. However, very little progress has been made in finding post-exposure prophylaxis for WEEV. Using the mouse WEEV model, DRDC Suffield has tested a novel strategy for developing such medical countermeasures. A modified, harmless human adenovirus has been used to ferry a gene encoding a potent antiviral protein, interferon alpha (IFN-α), into the body. In this study, DRDC Suffield has demonstrated that IFN-α was rapidly produced in mice after intramuscular (IM) injection of the adenovirus and that a single-dose IM injection provided post-exposure protection against the lethal challenge of WEEV. This pilot study provides insights into further development of medical countermeasures for post-exposure prophylaxis against WEEV and other viral biothreat agents.

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Keywords
Western equine encephalitis virus;WEEV;post-exposure;prophylaxis
Report Number
DRDC-SUFFIELD-TM-2009-211 — Technical Memorandum
Date of publication
01 Nov 2009
Number of Pages
34
DSTKIM No
CA044402
CANDIS No
804798
Format(s):
Electronic Document(PDF)

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