Molecular Typing of Bacillus anthracis isolates using MLVA-15

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Authors
  1. Stratilo, C.W.
  2. Bader, D.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
Bacillus anthracis, the etiological agent of anthrax, lacks character homoplasy and is genetically very monomorphic. Variable number tandem repeats (VNTR) are rapidly evolving stretches of DNA that can serve as markers for strain differentiation (molecular typing). A collection of B. anthracis strains were previously characterized using multiple-locus VNTR analysis (MLVA-8). This work describes the characterization of 31 strains by MLVA-15. MLVA-15 allowed these strains to be placed within a phylogenetic framework as with MLVA-8. MLVA-15 did allow higher resolution and discrimination than MLVA-8. While MLVA-15 allowed many strains to be discriminated from each other, several Canadian isolates were indistinguishable. This work allowed verification of strain designation within the DRDC Suffield collection and allowed for the development of a high throughput genetic typing capability at DRDC Suffield.

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Keywords
Bacillus anthracis;Genetic Typing;Identification of bacteria
Report Number
DRDC-SUFFIELD-TM-2013-124 — Technical Memorandum
Date of publication
01 Dec 2013
Number of Pages
32
DSTKIM No
CA044633
CANDIS No
804957
Format(s):
Electronic Document(PDF)

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