Human hair follicle transcriptome profiling – A minimally invasive tool to assess molecular adaptations upon low-volume, high-intensity interval training


  1. Zhang, J.
  2. Wallace, S.J.
  3. Shiu, M.Y.
  4. Smith, I.
  5. Rhind, S.G.
  6. Laglois, V.S.
Corporate Authors
Defence Research and Development Canada, Toronto Research Centre , Toronto ON (CAN);Royal Military Coll of Canada, Kingston ONT (CAN) Dept of Chemistry and Chemical Engineering
High-intensity interval training (HIIT) has become a popular fitness training approach under both civilian and military settings. Consisting of brief and intense exercise intervals, HIIT requires less time commitment yet is able to produce the consistent targeted physical adaptations as conventional endurance training. To effectively characterize and monitor HIIT-induced cellular and molecular responses, a highly accessible yet comprehensive biomarker discovery source is desirable. Both gene differential expression (DE) and gene set (GS) analyses were conducted using hair follicle transcriptome established from pre and postexercise subjects upon a 10 day HIIT program by RNA-Seq, Comparing between pre and posttraining groups, differentially expressed protein coding genes were identified. To interpret the functional significance of the DE results, a comprehensive GS analysis approach featuring multiple algorithms was used to enrich gene ontology (GO) terms and KEGG pathways. The GS analysis revealed enriched themes such as energy metabolism, cell proliferation/growth/survival, muscle adaptations, and cytokine-cytokine interaction, all of which have been previously proposed as HIIT responses. Moreover, related cell signaling pathways were also measured. Specifically, G-protein-mediated signal transduction, phosphatidylinositide 3-kinases (PI3K)- protein kinase B (PKB) and Janus kinase (JAK) - Signal Transducer and Activator of Transcription (STAT) signaling cascades were overrep
RNA-Seq;endurance training;muscle contraction;energy metabolism;miRNA
Report Number
DRDC-RDDC-2018-P134 — External Literature
Date of publication
01 May 2018
Number of Pages
Reprinted from
Physiological Report, vol 5, iss 23, e13534
Electronic Document(PDF)

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