Quantitative PCR analysis of the decay and recovery of biothreat agents during sample storage

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Authors
  1. Lee, W.E.
  2. Tang, T.
  3. Bader, D.E.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
Real-time quantitative polymerase chain reaction (PCR) is widely employed for detection and identification of genetic markers in DNA-containing samples. This report describes methods to use quantitative PCR in conjunction with reaction rate kinetics and thermodynamics to determine recovery of sample material from a commercially available storage medium, FTA® cards. The report shows practitioners of PCR a method to assess analyte recovery, plus the kinetic and thermodynamic parameters associated with sample decay during storage.

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Keywords
Real-time polymerase chain reaction kinetics
Report Number
DRDC-SUFFIELD-TM-2009-279 — Technical Memorandum
Date of publication
01 Dec 2009
Number of Pages
38
DSTKIM No
CA046056
CANDIS No
806532
Format(s):
Electronic Document(PDF)

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