Protein-nucleic acid (receptor-ligand) binding detection techniques

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Authors
  1. Sheibani, S.
  2. Chan, N.W.C.
Corporate Authors
Defence Research and Development Canada, Suffield Research Centre, Ralston AB (CAN)
Abstract
The key components of the Inform Project WBE 5.3 is on the development of a handheld biosensor to provide early warning to the Canadian Armed Forces (CAF) personnel for a potential biological threat. This technology is based on Toll-Like Receptors (TLRs) which are a class of proteins expressed by the innate immune system cells. TLRs recognize a specific component of pathogen through direct binding or via an intermediate molecule. Among the 10 TLRs that have been identified to date, TLRs 3, 7, 8 and 9 recognize various types of microbial nucleric acids. There are two main types of nucleic acids including deoxyribonucleric acid (DNA) and ribonucleic acid (ssRNA) viruses. Based on the Baltimore classification, viruses are classified into families depending on their type of nucleic acid and method of replication. The ssRNA viruses belong to class IV or V. A part of TLR biosensor project is on detection of ssRNA viruses using TLR7 and TLR8 through a binding assay. Therefore, the purpose of this Scientific Report is to recommend the best available technique to study the binding of TLR7 and TLR8 (receptor) with ssRNA virus (ligand) in the context of this project. To acheive this goal, in the first step, a literature review on the current techniques is required. Among a small set of current methods (labelling and label-free), Surface Plasmon Resonance (SPR) which is a label-free technique is recommended for the purpose of this project.

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Keywords
Protein;nucleic acid;ligand;ssRNA virus;TLR7;TLR8;detection technique
Report Number
DRDC-RDDC-2018-R027 — Scientific Report
Date of publication
01 Jun 2018
Number of Pages
21
DSTKIM No
CA046879
CANDIS No
807290
Format(s):
Electronic Document(PDF)

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