Electrophysiology and field recording in rat brain slices for traumatic brain injury research – Standard operating procedure

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Authors
  1. Barnes, J.
  2. Weiss, T.
  3. Wang, Y.
Corporate Authors
Defence Research and Development Canada, Suffield Research Centre, Ralston AB (CAN)
Abstract
Electrophysiology is the study of electrical properties of biological cells and tissues. It studies the flow of ions (ion current) in biological systems, including the brain. It involves measurements of changes in voltage or electric current on a wide variety of scales from single ion channel proteins to whole organs. Electrophysiological techniques are widely used to investigate mechanisms of physiological or pathological processes in a variety of organs, including the brain. To assist studies at DRDC – Suffield Research Centre in traumatic brain injury research, we have recently acquired an electrophysiology / patch clamp system. This system is being used to study the molecular mechanisms of traumatic brain injury, either caused by primary blast waves or concussion by weight drop in small laboratory animals. This Standard Operating Procedure (SOP) is design to guide the operation of this system to perform field recordings to study changes in Long Term Potentiation (LTP) and Long Term Depression (LTD). Both LTP and LTD are considered the molecular basis of learning and memory.

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Keywords
Traumatic Brain Injury (TBI);primary blast;electrophysiology
Report Number
DRDC-RDDC-2019-D111 — Reference Document
Date of publication
01 Aug 2019
Number of Pages
18
DSTKIM No
CA049886
CANDIS No
810729
Format(s):
Electronic Document(PDF)

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