Report on End of Year 1 Research – Improve Electrochemical Sensor Chip Performance and Establish Baseline Parameters for Aerosol Electrochemical Detection

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Authors
  1. Marenco, A.J.
  2. Singh, R.
  3. Renaud-Young M.
  4. Mayall, R.M.
  5. Birss, V.I.
Corporate Authors
Defence Research and Development Canada, Suffield Research Centre, Ralston AB (CAN);Calgary Univ, Calgary ALTA (CAN)
Abstract
This document reports on an electrochemical-based technology for the detection of harmful bacteria and bioaerosols. The focus of this work is the detection of Gram-negative bacteria with the ligand-binding domains of Toll-like receptor (TLR) immunoproteins tethered to an electrode surface. Specifically, this report describes experimental outcomes during the first option year of the contract (revisiting Tasks 5.1 and 5.2, and progress made towards Tasks 5.3, 5.4, and 5.5). For Tasks 5.1 and 5.2, we worked specifically on understanding and reducing signal drift (where the signal of the sensor changes over time, without contact with the target molecule), which has been a key technical challenge of this technology. Tasks 5.3 and 5.9 were focused on bioaerosols (airborne organisms and biomatter) and involved the development of sample collection techniques and analysis using the standard immunoassay for the detection of Gram-negative lipopolysaccharide toxic biomolecules (Limulus amoebocyte lysate). At a later time, samples analysed by these methods will be tested electrochemically. Task 5.4 explored the development of a Gram-positive bacterial sensor utilizing TLRs 1, 2 and 6. At this time, we have investigated the binding interactions of TLR2 and its bacterial targets via microscale thermophoresis and will soon test TLR2 as a working electrochemical sensor. Task 5.5 has focussed on our attempts to regenerate an electrochemical TLR sensor by removing the protein moiety or the enti
Keywords
Toll-like receptors;Electrochemical impedance spectroscopy;Bioaerosols;Gram-negative bacteria;Lipopolysaccharide;Biodetection;Self-assembled monolayers
Report Number
DRDC-RDDC-2019-C219 — Contract Report
Date of publication
01 Sep 2019
Number of Pages
47
DSTKIM No
CA050015
CANDIS No
810883
Format(s):
Electronic Document(PDF)

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