A System for the Radid Detection of Low Numbers of Viable Cells of Aerobacter Aerogenes

Authors
  1. White, L.A.
  2. MacLeod, R.A.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A sensitive method is presented for the rapid detection of resting cells of Aerobacter aerogenes in which the cells are labelled with 32P-orthophosphate. The uptake of this anion by the cells, as well as the sensitivity of the method, has been investigated and shown to be dependent upon several factors. An incubation medium composed of 1/100 M potassium ion, 5/1000 M magnesium ion, 1mg/ml of glucose and 1 Ci/ml of 32P04, buffered at pH 6.55 with 0.05M N-2-Hydroxyethyl-piperazine-N'-2-ethanesulfonic acid (HEPES), was found to stimulate optimum accumulation of 32P-orthophosphate. The temperature of incubation, incubation time, the concentration of unlabelled orthophosphate, as well as arsenate, and several metabolic inhibitors were found to affect the accumulation. The labelled cells were collected on a membrane filter, which had been previously boiled in glass distilled water, for measurement of the radioactivity accumulated. Under optimum conditions, as few as 20,000 viable cells
Keywords
Aerobacter aerogenes
Report Number
DRES-TP-374 — Technical Paper
Date of publication
15 Jan 1971
Number of Pages
28
DSTKIM No
71-02390
CANDIS No
8737
Format(s):
Hardcopy;Originator's fiche received by DSIS

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