QUANTITATIVE STUDIES OF CYTOLOGICAL DAMAGE IN MOUSE TESTIS PRODUCED BY EXPOSURE TO HEAT

Authors
  1. Cairnie, A.B.
  2. Leach, K.E.
Corporate Authors
Defence Research Establishment Ottawa, Ottawa ONT (CAN)
Abstract
Damage in gonadal cells produced by exposure of the hindquarters of conscious male mice to water at temperatures up to 43C was studied by measuring the percentage of testicular cells which do not exclude trypan blue 4 h after exposure, sperm count up to ten wk later, and the percentage of morphologically abnormal sperm up to ten wk later. It was found that the percentage of cells stained with trypan blue was significantly increased by exposure for 0.5 h at 41C or 2 h at 37C. Sperm count and the percentage of abnormal sperm were affected by 0.5 h at 43C or 2 h at 40C, but not by lower temperatures. Since there is a high incidence of abnormal sperm after some heat treatments, the use of this assay for mutagen screening should be approached with caution.
Report Number
821 —
Date of publication
01 Jan 1980
Number of Pages
10
Reprinted from
Can J Genet Cytol, vol 22, 1980, p 93-102
DSTKIM No
80-02058
CANDIS No
89745
Format(s):
Hardcopy;Originator's fiche received by DSIS

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