IMMUNE RESPONSE MEDIATED BY LIPOSOME-ASSOCIATED PROTEIN ANTIGENS. IV. MODULATION OF ANTIBODY FORMATION BY VESICLE-ENCAPSULATED METHOTREXATE

Authors
  1. Shek, P.N.
  2. Lopez, N.G.
  3. Heath, T.D.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
Large unilamellar reverse-phase evaporation vesicles (REV) were used as a heterobifunctional carrier for a pharmacologically active agent and a protein antigen. Methotrexate (MTX) was encapsulated in the hydrophilic compartment of liposomal REV with or without surface-conjugated bovine serum albumin (BSA) antigen. The administration of MTX encapsulated within BSA-coated vesicles (MTX.REV-BSA) could either enhance or depress the anti-BSA plaque-forming cell (PFC) response in mice. On the other hand, the administration of MTX entrapped in plain vesicles (MTX.REV) produced essentially a suppressive effect onthe PFC response stimulated by the simultaneous injection of a separate antigen, e.g. vesicle-conjugated BSA (REV-BSA). The suppression of the antibody response occurred, whether MTX.REV was injected 1 day before, during, or 1 day after the immunization with an antigen. TRUNCATED
Report Number
DCIEM-85-P-41 — Research Paper
Date of publication
15 Sep 1985
Number of Pages
5
Reprinted from
Immunology, no 57, 1986, p 153-157
DSTKIM No
86-01955
CANDIS No
96677
Format(s):
Hardcopy;Originator's fiche received by DSIS

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