MEASUREMENT OF MACROPHAGE ACTIVATION BY CHEMILUMINESCENCE

Authors
  1. Lippe, R.
  2. Markham, R.J.F.
  3. Erhardt, N.P.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Chemiluminescence (CL) stimulated by the phagocytosis of yeast particles was used to monitor metabolic activity of murine macrophages obtained by either peritoneal or bronchoalveolar lavage. Intraperitoneal injection of the macrophage activator lipopolysaccharide (LPS) from Escherichia coli led to the appearance of macrophages in the peritoneal lavage fluid which had enhanced CL when compared to macrophages from control animals injected with saline or phosphate buffered saline. Intraperitoneal injection of LPS, however, was not able to enhance the CL activity of cells obtained by bronchoalveolar lavage whereas intratracheal instillation of LPS directly into the lung did elicit a population of cells with increased chemiluminescence. Thus it appears that CL is a useful measure of macrophage activation.
Report Number
DRES-M-1166 — Memorandum
Date of publication
15 May 1986
Number of Pages
24
DSTKIM No
86-02344
CANDIS No
97058
Format(s):
Hardcopy;Originator's fiche received by DSIS

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