GC/MS AND EMIT ANALYSES FOR DELTA 9-TETRAHYDROCANNABINOL METABOLITES IN PLASMA AND URINE OF HUMAN SUBJECTS

Authors
  1. McBurney, L.J.
  2. Bobbie, B.A.
  3. Sepp, L.A.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
Ten male subjects smoked placebo marijuana cigarettes spiked with delta 9 tetrahydrocannabinol (THC) at 150 micrograms g/kg body weight. Plasma and urine samples were collected for 22 hr after smoking. Total cross-reacting cannabinoids were measured by immunoassay (EMIT) and THC and major metabolities were identified and quantitated by gas chromatographic/mass spectrometric (GC/MS) procedures. In plasma, THC concentration provided the best indication of recent (<6 hr) smoking. In enzyme-hydrolyzed urine, 8 Beta, 11-dihydroxy-THC at high concentration was identified in the earliest voidings, falling rapidly to the limit of detection before 22 hr in most subjects. Levels above 15 to 20 ng/mL were indicative of use within the previous 4 to 6 hr.
Report Number
DCIEM-85-P-44 — Research Paper
Date of publication
01 Jan 1986
Number of Pages
9
Reprinted from
J of Analytical Toxicology, vol 10, 1986, p 56-64
DSTKIM No
86-02460
CANDIS No
97170
Format(s):
Hardcopy;Originator's fiche received by DSIS

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