ENZYME IMMUNOASSAY SYSTEMS UTILIZING POLYCLONAL ANTIBODY AS CAPTURE REAGENTS IN IDENTIFICATION OF NEWCASTLE DISEASE VIRUS ANTIGENS

Authors
  1. Fulton, R.E.
  2. Erhardt, N.P.
  3. Frank, R.I.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Four enzyme immunoassay systems, which utilize polyclonal antibodies as capture reagents attached to the wells of polystyrene microtiter plates, have been developed for the identification and quantitation of Newcastle Disease Virus, or its antigens, in environmental samples. Reagents were standardized and, using purified virus protein, the theoretical lower limits of sensitivity of each system was determined by dilution end-point. The enzyme immunoassay systems developed were 1000 to 2000 times more sensitive than the conventional hemagglutination assay and could detect virus protein at a concentration of 1 - 2 ng per well, or 3 - 9 ng per ml of test sample. Tests were simple to perform and, once captured antibodies had been adsorbed to the solid phase, results could be obtained in 3 - 4 h. The techniques described are potentially adaptable to large scale automation for use in a laboratory setting or, alternatively, may be adapted for manual use in a field scenario.
Report Number
DRES-435 — Final Report
Date of publication
15 Jun 1986
Number of Pages
48
DSTKIM No
86-02958
CANDIS No
97654
Format(s):
Hardcopy;Originator's fiche received by DSIS

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